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Image Search Results
Journal: Archives of Toxicology
Article Title: Establishment of a human 3D in vitro liver-bone model as a potential system for drug toxicity screening
doi: 10.1007/s00204-024-03899-9
Figure Lengend Snippet: The antibodies used for dot blot
Article Snippet:
Techniques:
Journal: Archives of Toxicology
Article Title: Establishment of a human 3D in vitro liver-bone model as a potential system for drug toxicity screening
doi: 10.1007/s00204-024-03899-9
Figure Lengend Snippet: DCFH-DA assay was used to detect the ROS levels in liver spheroids. Ellman assay was used to detect the GSH and GSSG levels in liver spheroids. The expression of secreted IL-6, sRANKL, and OPG protein levels in the liver-bone system supernatant were measured by dot blot. a After acute exposure to diclofenac, the ROS levels in liver spheroids on day 7, H 2 O 2 stimulation as a positive control. b After acute exposure to diclofenac, the ratio of GSH and GSSG in liver spheroids on day 7. c With the stimulation of 3–6 µM diclofenac, the IL-6 protein levels in supernatant on day 7, and the representative image of dot blot. d With the stimulation of 3–6 µM diclofenac, the sRANKL protein levels in supernatant on day 7, and the representative image of dot blot. e With the stimulation of 3–6 µM diclofenac, the OPG protein levels in supernatant on day 7, and the representative image of dot blot. f The ratio of sRANKL and OPG in the liver-bone co-culture system. The Kruskal–Wallis test followed by Dunn’s multiple comparison test was used to determine statistical differences. Data are presented as means ± SEM, and the significance is shown as * p < 0.05, *** p < 0.001, and **** p < 0.0001 vs. Control group. N ≥ 3, n ≥ 2
Article Snippet:
Techniques: DCFH-DA Assay, Expressing, Dot Blot, Positive Control, Co-Culture Assay, Comparison, Control